[new port] biology/kallisto: Quantify abundances of transcripts from RNA-Seq data

Approved by: jrm (mentor)
Differential Revision:  https://reviews.freebsd.org/D13328
This commit is contained in:
Jason W. Bacon 2017-12-02 20:44:28 +00:00
parent 40fc605e9d
commit 429caa13b5
Notes: svn2git 2021-03-31 03:12:20 +00:00
svn path=/head/; revision=455382
5 changed files with 91 additions and 0 deletions

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@ -41,6 +41,7 @@
SUBDIR += iqtree
SUBDIR += jalview
SUBDIR += jellyfish
SUBDIR += kallisto
SUBDIR += lagan
SUBDIR += lamarc
SUBDIR += libgtextutils

33
biology/kallisto/Makefile Normal file
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# $FreeBSD$
PORTNAME= kallisto
DISTVERSIONPREFIX= v
DISTVERSION= 0.43.1
CATEGORIES= biology
MAINTAINER= jwb@FreeBSD.org
COMMENT= Quantify abundances of transcripts from RNA-Seq data
LICENSE= BSD2CLAUSE
LICENSE_FILE= ${WRKSRC}/license.txt
LIB_DEPENDS= libhdf5.so:science/hdf5 \
libsz.so:science/szip
USES= cmake
USE_GITHUB= yes
GH_ACCOUNT= pachterlab
SUB_FILES= kallisto-test
PORTEXAMPLES= *
PLIST_FILES= bin/kallisto
EXAMPLES_PLIST_FILES= bin/kallisto-test
OPTIONS_DEFINE= EXAMPLES
post-install-EXAMPLES-on:
${INSTALL_SCRIPT} ${WRKDIR}/kallisto-test ${STAGEDIR}${PREFIX}/bin
cd ${WRKSRC}/test && ${COPYTREE_SHARE} . ${STAGEDIR}${EXAMPLESDIR}
.include <bsd.port.mk>

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TIMESTAMP = 1512166931
SHA256 (pachterlab-kallisto-v0.43.1_GH0.tar.gz) = 2164938c2c61c04e338c4c132cf749f56d39e6f0b4c517121bca1fbc218e430e
SIZE (pachterlab-kallisto-v0.43.1_GH0.tar.gz) = 1067076

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#!/bin/sh
##########################################################################
# Script description:
#
# Arguments:
#
# Returns:
#
# History:
# Date Name Modification
# 2016-03-21 Charlie & Begin
##########################################################################
usage()
{
printf "Usage: $0 test-directory\n"
exit 1
}
##########################################################################
# Main
##########################################################################
if [ $# != 1 ]; then
usage
fi
dir="$1"
if [ -e "$dir" ]; then
printf "$dir already exists. Remove it first or choose a different name.\n"
exit 1
fi
cp -iR %%EXAMPLESDIR%% "$dir"
cd "$dir"
kallisto index -i transcripts.idx transcripts.fasta.gz
kallisto quant -i transcripts.idx -o output -b 100 reads_1.fastq.gz reads_2.fastq.gz
ls -l output
more output/abundance.tsv
cat << EOM
See https://pachterlab.github.io/kallisto/starting.html for instructions
on interpreting the output above.
EOM

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Kallisto is a program for quantifying abundances of transcripts from RNA-Seq
data, or more generally of target sequences using high-throughput sequencing
reads. It is based on the novel idea of pseudoalignment for rapidly determining
the compatibility of reads with targets, without the need for alignment.
WWW: https://pachterlab.github.io/kallisto/about.html